Enzymes and Inhibitors
Thermo Scientific™ Bsp119I (BstBI) (10 U/μL)
Cut at TT^CGAA sites with Bsp119I (BstBI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AsuII, Bpu14I, BspT104I, BstBI, Csp45I, NspV, SfuIm).
Thermo Scientific™ AloI (2 U/μL)
Cut at ^(7/12-13)GAAC(N)6TCC(12-13/7)^ sites with AloI restriction enzyme, which performs best at 30°C in R buffer.
Thermo Scientific™ BpiI (BbsI) (10 U/μL)
Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).
Thermo Scientific™ Eco130I (StyI) (10 U/μL)
Cut at C^CWWGG sites with Eco130I (StyI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BssT1I, EcoT14I, ErhI, StyI).
Invitrogen™ Anza™ 12 XbaI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ Klenow Fragment (10 U/μL)
Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.
Thermo Scientific™ Terminal Deoxynucleotidyl Transferase (20 U/μL)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the template-independent addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules.
Thermo Scientific™ Exonuclease III (200 U/μL)
Digest double-stranded DNA or DNA-RNA hybrids with Exonuclease III is a 3'–>5' exonuclease.
Thermo Scientific™ FspAI (5 U/μL)
The FspAI restriction enzyme recognizes RTGC^GCAY sites and cuts best at 37°C in O buffer.
Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Invitrogen™ X-Gal (5-Bromo-4-Chloro-3-Indolyl β-D-Galactopyranoside)
Widely used chromogenic substrate for β-galactosidase
Invitrogen™ Rhodamine 110, bis-(p-Tosyl-L-Glycyl-L-Prolyl-L-Arginine Amide)
This Molecular Probes™ bisamide derivative of rhodamine 110 (R110) is a sensitive and selective substrate for assaying proteases in solution or inside living cells.
Invitrogen™ LR Clonase™ II Plus enzyme
Mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor), and Xis (Excisionase) enzymes.
Invitrogen™ Anza™ 93 HpaII
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.